Sökning: "affinity precipitation"

Visar resultat 1 - 5 av 23 avhandlingar innehållade orden affinity precipitation.

1. 1. Specific Precipitation of Biomolecules Using Synthetic Polymers

   Författare :Per-Olof Wahlund; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Biotechnology; Polymerteknik; RNA; plasmid DNA; DNA; polyelectrolyte complexes; synthetic polymers; precipitation; affinity precipotation; Bioteknik; Polymer technology; biopolymers;

   Sammanfattning : Purification is a very important stage in biotechnological processes. The traditionally used precipitation techniques are generally nonspecific and thus one or more chromatographic steps are normally required. There is thus a need for new approaches to improve existing techniques. LÄS MER

3. 2. Metal Affinity Peptides in Biotechnological Applications As tools in protein purification, protein stabilisation, metal quantification and metal tolerance of bacteria and plants

   Författare :Malin Mejàre; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; cadmium; metal quantification; fusion tag; metal affinity tag; phage display; peptide library; metal tolerance; metal resistance; metal accumulation; bioremediation; transgenic plant; tobacco; potato; protein purification; immobilised metal affinity chromatography; metal affinity precipitation; metal detection; protein stabilisation; Bioteknik; Biotechnology; Biokemi; Metabolism; Biochemistry; metal ion;

   Sammanfattning : Metal affinity tags have been fused to proteins and demonstrated to function as tools in protein purification, protein stabilisation, quantitative metal analysis and metal tolerance of bacteria and plants. In biotechnological applications proteins frequently have to function in nonnatural environments with harsh conditions which impose requirements for high stability on the proteins. LÄS MER

4. 3. Genetically Engineered Plants: Improved stress tolerance, growth and facilitated protein purification

   Författare :Gösta Lilius; Tillämpad biokemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; glycine betaine; gene; freezing; flooding; drought; dicoteledones; desiccation; compatible solutes; cold; choline dehydrogenase; chill; CDH; betaine; betA; alkaloids anoxia; affinity purification; affinity tags; handles; hemoglobin; hypoxia; low temperatu; Biotechnology; Bioteknik;

   Sammanfattning : Heterologous genes were expressed in plants in which the overall goal was to increase the value of the final crop. Various aspects were explored and evaluated, such as improving the stress tolerance, enhancing the availability of oxygen in growing cells and producing rare proteins with increased degrees of purity. LÄS MER

5. 4. Polyelectrolytes and Polycomplexes in Biotechnology

   Författare :Maria Dainiak; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; expanded bed adsorption.; affinity precipitation; Fab fragments; monoclonal antibodies; glyceraldehyde-3-phosphate dehydrogenase; misfolded protein; chaperone; poly N-ethyl-4-vinylpyridinium bromide ; poly methacrylic acid ; poly acrylic acid ; Key words: polyelectrolyte; polyelectrolyte complexes; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi;

   Sammanfattning : Polyelectrolytes and polyelectrolyte complexes (PECs) are characterized by many useful properties that endow them with high potential in different areas of biotechnology. This thesis describes the development and analyses of some new systems and methods utilizing poly(acrylic acid) (PAA), poly(methacrylic acid) (PMA), poly (N-ethyl-4-vinylpyridinium bromide) (PEVP) and PMA-PEVP complexes. LÄS MER

7. 5. Immuno-affinity interactions in sample preparation and analysis

   Författare :Madalina Tudorache; Centrum för analys och syntes; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; immuno supported liquid membrane ISLM extraction; sample preparation; immunoassay; immuno-affinity interaction; antibody; Analytical chemistry; Analytisk kemi;

   Sammanfattning : Immuno-affinity interactions (antibody-antigen) belong, together with enzyme-inhibitor/substrate/coenzyme; oligosaccharide-lectin; DNA/RNA-protein interactions etc, to the class of bio-recognition. Immuno-affinity is widely used for analysis in immunoassays with numerous applications in a variety fields (i.e. LÄS MER