Sökning: "RNA origami"
Hittade 4 avhandlingar innehållade orden RNA origami.
1. RNA in model lipid membranes - interactions in bulk and at surfaces
Sammanfattning : The work presented in the thesis “RNA in model lipid membranes- interactions in bulk and at surfaces” aims to characterize the interaction between RNA and model membranes with different properties as well as to determine if ssRNA associate with model membranes in different ways. Several techniques have been used to characterize these systems, including quartz crystal microbalance with dissipation monitoring (QCM-D), Langmuir film balance in combination with neutron reflectometry and fluorescence microscopy as well as differential scanning calorimetry (DSC) and isothermal titration calorimetry (ITC). LÄS MER
2. Applications of DNA origami encoded nanoscale patterns
Sammanfattning : It was almost four decades ago when the recognition of DNA’s potential use as a programmable, self-assembling building material for nanostructures led to the birth and rapid expansion of the field of DNA nanotechnology, but it was two decades later when the development of the DNA origami technique initiated the widespread use of DNA based nanoconstructs through the simplification of the design process and the reduction of the required control over the quality and stoichiometry of the assembly components by using a single-stranded “scaffold” DNA and a set of “staple” oligonucleotides that “fold” the mentioned scaffold DNA into a predesigned shape by binding different regions of the scaffold strand together. This robust approach not only permitted the construction of intricate two- and three-dimensional structures, but it also allowed the design and fabrication of molecular patterns with unprecedented accuracy as each functionalizible component’s relative position in the DNA origami structure is known to nanometer precision. LÄS MER
3. Nucleic acid tools for detection and characterization of biological systems
Sammanfattning : Nucleic acids, DNA and RNA, are naturally occurring biopolymers synthetized by cells to store and propagate genetic information. They can be found in eukaryotic cells, bacteria, archaea and viruses and, thanks to the development of synthetic chemistry techniques, they can be synthetized with relative ease on demand in the laboratory. LÄS MER
4. Developing molecular tools for probing and modulating genomic spatial adjacency
Sammanfattning : In addition to the vast information encoded in DNA sequence, the genome has physical features that are also essential for its function, including its organization in threedimensional space. The development of high-throughput technology has greatly advanced our understanding of the spatial organization of the genome but has also raised more questions. LÄS MER
