Sökning: "RFLP"

Visar resultat 1 - 5 av 50 avhandlingar innehållade ordet RFLP.

1. 1. Characterization and Use of a Multiplex PCR-based System: Random amplified Polymorphic DNA

   Författare :Christer Halldén; Malmö Klinisk kemi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; DNA mixtures; competition; graphical genotypes; bulked segregant analysis; linkage map; genetic distance; RFLP markers; RAPD markers; oilseed rape; sugar beet; Genetics; cytogenetics; Genetik; cytogenetik;

   Sammanfattning : Random amplified polymorhic DNA (RAPD) is a PCR-based marker system that makes use of universal sets of short oligonucleotides that amplify random DNA fragments under low-stringency conditions. The RAPD marker system has found widespread use and possesses a number of very positive features, being a multiplex and universal system. LÄS MER

3. 2. Microbiota of the alimentary tract of children - implications for allergy and inflammatory bowel disease

   Författare :Fei Sjöberg; Göteborgs universitet; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; gut flora; child; oral flora; allergy; duodenum; inflammatory bowel disease; culture; T-RFLP; pyrosequencing;

   Sammanfattning : Allergy, which is the most common chronic disease in Swedish children and adolescents, is associated with a high standard of living and Western lifestyle. According to the hygiene hypothesis, allergy is due to inadequate stimulation of the immune system by microbes during early childhood, leading to failed maturation of the immune system. LÄS MER

4. 3. Characterization of the Intestinal Microbiota by DNA-Based Methods

   Författare :Mei Wang; Livsmedelsteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; mycology; Mikrobiologi; bakteriologi; virologi; mykologi; bacteriology; virology; Microbiology; Ulcerative colitis; T-RFLP; 16S rRNA; Brachyspira; Microbiota; Bacterial diversity; Bacterial translocation; Translocating bacteria;

   Sammanfattning : The intestinal flora and the bacteria found in the extraintestinal sites were studied in liver injury rats, with or without Lactobacillus plantarum 299v pre-treatment. The dominating intestinal flora and translocating bacteria were typed by RAPD and identified by 16S rDNA sequencing. LÄS MER

5. 4. Composition and dynamics of the bacterial community in aerobic granular sludge reactors

   Författare :Enikö Barbara Szabo; Chalmers tekniska högskola; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; qPCR; sequencing batch reactors; microbial community composition; T-RFLP; functional groups; Illumina MiSeq; wastewater; aerobic granular sludge; nitrogen removal; population dynamics;

   Sammanfattning : The aerobic granular sludge (AGS) technology is probably the future standard for wastewater treatment, due to its low footprint and low energy consumption. Although achieving granulation is usually not a challenge anymore, our understanding of the community assembly during start-up, and of the microbial ecology of these reactors in general, is incomplete. LÄS MER

7. 5. Patterns of geographic variation in Silene section Elisanthe (Caryophyllaceae): hybridization and migrational history

   Författare :Louise Hathaway; Biologiska institutionen; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; chemotaxonomy. Physiology of nonvascular plants; phytogeography; morphology; outbreeding depression; Systematic botany; taxonomy; ITS; fitnes; inbreeding depression; AFLP; PCR-RFLP; cpDNA; gene flow; postglacial migration; geographic variation; hybridization; Silene latifolia; Silene section Elisanthe; Silene dioica; Systematisk botanik; taxonomi; morfologi; kemotaxonomi. Växters fysiologi inte kärlväxter .;

   Sammanfattning : Large-scale patterns of genetic variation in chloroplast (cp) and nuclear DNA in the widespread European herb species Silene latifolia and S. dioica were investigated using cpPCR-RFLPs, non-coding cpDNA sequences, AFLPs and sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA). LÄS MER