Sökning: "Plasma membrane"

Visar resultat 16 - 20 av 560 avhandlingar innehållade orden Plasma membrane.

  1. 16. Cell-penetrating peptides in model membrane systems : Interaction, structure induction and membrane effects

    Författare :Mazin Magzoub; Astrid Gräslund; Steven Dowdy; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Molecular biophysics; Molekylär biofysik;

    Sammanfattning : Despite continuing advances in the development of macromolecules, including peptides, proteins, and oligonucleotides, for therapeutic purposes, the successful application of these hydrophilic molecules has so far been hampered by their inability to efficiently traverse the plasma membrane. The discovery of a class of peptides (cell-penetrating peptides, CPPs) with the ability to mediate the non-invasive and efficient import of a whole host of cargoes, both in vitro and in vivo, has provided a new means by which the problem associated with cellular delivery can be circumvented. LÄS MER

  2. 17. The surface complex of Euglena gracilis. Involvement in cellular movements and biochemical and molecular analyses of the components

    Författare :Silja K Petersen-Mahrt; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; UV-B radiation; tubulin; surface complex; plasma membrane; phylogeny; phototaxis; nitrogen deficiency; membrane skeleton; Euglena gracilis; actin; cell shape; Physiological biophysics; Växtfysiologi;

    Sammanfattning : The flagellate Euglena gracilis shows rhythms in phototaxis and cell shape changes between oblong and round. Artificial UV-B radiation of 0.5 W m-2 caused a loss in phototaxis, but the peak of activity occurred at the same time as for control cells. LÄS MER

  3. 18. Membrane protein proteomics - Novel method for membrane protein identification and quantification

    Författare :Maria Bentz; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biokemisk teknik; mebran; steroider; Lipider; membranes; steroids; Lipids; enzymologi; Proteiner; Membrane proteins; Proteomics; Proteinase K; N-terminal labelling; Relative Quantitation; Database search; Cancer; Cytology; oncology; cancerology; Cytologi; onkologi; cancer; enzymology; Proteins; Biochemical technology;

    Sammanfattning : Membrane proteins are fairly refractory to digestion especially by trypsin. Less specific proteases like elastase and pepsin are much more effective. However database searching using non-tryptic peptides is much less effective due to the lack of charge localisation at the N- and C-termini and the absence of sequence specificity. LÄS MER

  4. 19. Blue native polyacrylamide gel electrophoresis - a functional approach to plant plasma membrane proteome studies

    Författare :Jonas Kjell; Biologiska institutionen; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; dodecyl maltoside; BN-PAGE; plant plasma membrane; protein complexes;

    Sammanfattning : In this thesis, I have shown that a method called BN-PAGE (a kind of native gel electrophoresis) can be used for studying the protein composition in the plasma membrane of plants. The method enables the study of native (in the functional units) hydrophobic protein complexes, i.e. LÄS MER

  5. 20. Plant Membrane Proteomics - using isolated membranes and proteins to compare and quantify subproteomes

    Författare :Katja Bernfur; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mass spectrometry; quantification; proteomics; metabolic labeling; plasma membrane;

    Sammanfattning : Membrane proteins are particularly important to characterize, since they are involved in cellular processes of utmost importance in control and regulation of cells, such as transport across membranes, signal transduction and photosynthesis. The word "proteome" means all proteins expressed by a e.g. LÄS MER