Sökning: "PCR PRIMERS"
Visar resultat 1 - 5 av 60 avhandlingar innehållade orden PCR PRIMERS.
1. Detection of human papillomavirus : a study of normal cells, cervical intraepithelial neoplasia and cancer of the uterine cervix
Sammanfattning : Human papillomavirus (HPV) infections of the genital tract are now recognized to be among the most prevalent sexually transmitted diseases and also a contributing factor to some cancers of the lower genital tract of women and men. Presence of HPV in a clinical specimen is confined to detection of the HPV genome by DNA hybridization techniques. LÄS MER
2. The ubiquity and type diversity of papillomaviruses in normal skin
Sammanfattning : Papillomaviruses are a diverse, epitheliotropic group of viruses. Some genotypes are the main cause of anogenital cancer in humans; others can induce skin lesions. In order to investigate the occurrence of subclinical skin infections with human papillomavirus (HPV), a pair of degenerate primers (FAP59/64) was designed. LÄS MER
3. Studies on genital ulcer disease in Tanzania. Aetiology with special reference to Haemophilus ducreyi, association with human immunodeficiecy virus infection, and human papilloma virus
Sammanfattning : Genital ulcer diseases (GUD) caused by Haemophilus ducreyi, Treponema pallidum and herpessimplex type 2 (HSV-2) are an important health problem in many developing countries. Ulcerativesexually transmitted infections (STIs) have been associated with increased acquisition andtransmission of human immunodeficiency virus type 1 (HIV-1). LÄS MER
4. Resistance to neuraminidase inhibitors in influenza A virus isolated from mallards
Sammanfattning : Influenza A virus belongs to the Orothomyxoviridae family of viruses and is one of the most common pathogens that cause infections of the respiratory tract. The aim of this thesis was to investigate if neuraminidase inhibitor (NAI) Tamiflu® (oseltamivir, OC) and Relenza® (zanamivir, ZA) - related resistance mutations exist in the neuraminidase (NA) gene of viruses collected from wild birds. LÄS MER
5. Detection and Quantification of Variable Viral RNA by Real-Time PCR Assays
Sammanfattning : As the area of nucleic acid based technologies develops, so will our understanding of how structural variations in DNA and RNA pathogens are associated with disease. The overall goal of this thesis is the development of broadly targeted measurement techniques for variable viral RNA by Real-Time PCR (here referred to as quantitative reverse transcriptase PCR, QRT-PCR). LÄS MER