Sökning: "Label-free"

Visar resultat 1 - 5 av 85 avhandlingar innehållade ordet Label-free.

  1. 1. Quality Control for high-throughput Quantitative Proteomics - Harnessing the potential of label-free LC-MS

    Författare :Marianne Sandin; Institutionen för immunteknologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteomics; mass spectrometry; quality control; data analysis; label-free; feature detection; alignment; biomarker discovery; separation techniques;

    Sammanfattning : Multiplex protein quantification, proteomics, is essential for uncovering new biomarkers and understanding biology. Liquid chromatography coupled to mass spectrometry (LC-MS) is the predominant technique for these measurements. LÄS MER

  3. 2. Label-free processing of stem cell preparations by acoustophoresis

    Författare :Franziska Olm; Avdelningen för molekylär hematologi; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; acoustophoresis; label-free cell separation; ultrasound; microfluidics; stem cell processing; CTC separation;

    Sammanfattning : The first bone marrow transplant in a human was performed in 1959, providing evidence that cells can be used for transplantation and treatment, revealing that healing capacities lie within the human body that ought to be understood. Since then hematopoietic stem cell transplantation has developed as a standard treatment for many cancers besides other malignancies. LÄS MER

  4. 3. Algorithmic discovery, development and personalized selection of higher-order drug cocktails : A label-free live-cell imaging & secretomics approach

    Författare :Efthymia Chantzi; Mats G Gustafsson; Ulf Hammerling; Andreas Bender; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; drug combination discovery and development; personalized pharmacotherapy; higher-order drug combination analysis; quantitative label-free live-cell imaging; secretomics; cell-cell communication; deep learning; convolutional neural networks; data mining; MapReduce; resampling; generalized highest single agent; COMBImageDL; COMBSecretomics; Bioinformatics; Bioinformatik; Pharmacology; Farmakologi; Engineering Science; Teknisk fysik;

    Sammanfattning : An upward trend in clinical pharmacology is the use of multiple drugs to combat complex and co-occurring diseases due to better efficacy, decreased toxicity and reduced risk of evolving resistance. Despite high late-stage attrition rates and the need for multi drug treatments, most drug discovery and development efforts are still mainly focused on new one-size-fits-all monotherapies. LÄS MER

  5. 4. Towards High-Throughput Phenotypic and Systemic Profiling of in vitro Growing Cell Populations using Label-Free Microscopy and Spectroscopy : Applications in Cancer Pharmacology

    Författare :Obaid Aftab; Mats Gustafsson; Neil Carragher; Uppsala universitet; []
    Nyckelord :label free vesicle detector; high-throughput; phase contrast microscopy; Library of Pharmacologically Active Compounds; High Content Screening; fluorometric microculture cytotoxicity assay; nuclear magnetic resonance; mass spectrometry;

    Sammanfattning : Modern techniques like automated microscopy and spectroscopy now make it possible to study quantitatively, across multiple phenotypic and molecular parameters, how cell populations are affected by different treatments and/or environmental disturbances. As the technology development at the instrument level often is ahead of the data analytical tools and the scientific questions, there is a large and growing need for computational algorithms enabling desired data analysis. LÄS MER

  7. 5. Multiparametric Optical Characterization of Biological Nanoparticles using Evanescent Field Sensing

    Författare :Mattias Sjöberg; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein corona; single-particle; liposomes; SPR; lipid nanoparticles; Waveguide scattering microscopy; label-free; mass quantification.;

    Sammanfattning : In light of the increasingly realized dependence of many biological functions on nanoscopic supramolecular assemblies, also including novel biotechnological applications, there is a need for advanced analysis methods capable of accurately quantifying different characteristics of these elusive entities. The prime aim of this thesis is the development and utilization of surface-based bioanalytical sensing methods for quantitative characterization of biological nanoparticles. LÄS MER