Sökning: "Klenow-polymerase"

Hittade 3 avhandlingar innehållade ordet Klenow-polymerase.

  1. 1. Effects of fusion tags on protein partitioning In aqueous two-phase systems and use in primary protein recovery

    Författare :Cynthia Hassinen; KTH; []
    Nyckelord :E. coli; Aqueous two-phase systems; Fusion proteins; Hydrophobic interaction chromatography; Expanded bed adsorption; Beta-galactosidase; Klenow polymerase; Z-domain; Peptide tags;

    Sammanfattning : The two techniques aqueoustwo-phase partitioning and expanded bed adsorption that bothare suitable for primary protein recovery were studied. Most ofthe work was focused on partition in aqueous two-phase systemsand in particular on the possibility to effect the partitionbehaviour by fusion of short peptide tags or protein domains tothe target protein. LÄS MER

  3. 2. Advancements in Firefly Luciferase-Based Assays and Pyrosequencing Technology

    Författare :Jonas Eriksson; KTH; []
    Nyckelord :bioluminescence; osmolytes; glycine betaine; thermostability; firefly luciferase; inorganic pyrophosphatase; inorganic pyrophosphate; Pyrosequencing technology; secondary DNA-structures; Sequenase; Klenow-polymerase; reaction rates; temperature;

    Sammanfattning : Pyrosequencing is a new DNA sequencing method relying on thesequencing-by-synthesis principle and bioluminometric detectionof nucleotide incorporation events. The objective of thisthesis was improvement of the Pyrosequencing method byincreasing the thermal stability of firefly luciferase, and byintroducing an alternative DNA polymerase and a new nucleotideanalog. LÄS MER

  4. 3. Method development and applications of Pyrosequencing technology

    Författare :Baback Gharizadeh; KTH; []
    Nyckelord :apyrase; bacterial identification; dATP?S; EST sequencing; fungal identification; human papillomavirus HPV ; microbial and viral typing; multiple sequencing primer method; Pyrosequencing technology; Sequenase; single-stranded DNA-binding protein SS;

    Sammanfattning : The ability to determine nucleic acid sequences is one ofthe most important platforms for the detailed study ofbiological systems. Pyrosequencing technology is a relativelynovel DNA sequencing technique with multifaceted uniquecharacteristics, adjustable to different strategies, formatsand instrumentations. LÄS MER