Sökning: "His 6 tag"

Hittade 5 avhandlingar innehållade orden His 6 tag.

  1. 1. The Membrane-Spanning Domain of Complex I Investigated with Fusion Protein Techniques

    Författare :Maria Trane; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NuoN; NuoM; NuoL; covalent bound heme; Bacillus subtilis; Escherichia coli; membrane protein; His-tag; Cytochrome c; NADH:quinone oxidureductase; fusion protein;

    Sammanfattning : NADH:quinone oxidoreductase, or complex I, is a large, complex and poorly understood bioenergetic enzyme in the respiratory chain of living organisms. The enzyme has a conserved core structure, comprising fourteen protein subunits: Seven subunits protrude from the membrane and contain a flavin prosthetic group and eight iron-sulfur clusters that guide electrons from the oxidation of NADH towards the quinone binding site(s). LÄS MER

  3. 2. Improving selectivity in protein extraction by subtle interactions and environmental changes

    Författare :Sheryl Fernandes; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

    Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER

  4. 3. Ribosomal Stalk Protein L12 : Structure, Function and Application

    Författare :Chandra Sekhar Mandava; Suparna Sanyal; Knud Nierhaus; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Ribosome; protein synthesis; L12 dimer; initiation; elongation; G factors; and His-tag; Molecular biology; Molekylärbiologi; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Sammanfattning : Ribosomal stalk proteins are known to play important role in protein synthesis. The ‘stalk’, an extended structure on the large subunit of the ribosome is composed mainly of two to three dimers of L12 and one L10 protein, which forms the base of the stalk. In E. LÄS MER

  5. 4. A radical view of haemoglobin - protein engineering and characterisation

    Författare :Marie Grey; Tillämpad biokemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; His-Gln tagging; protein purification; blood substitutes; radical scavenging; superoxide dismutase; E. coli; diglobin; Haemoglobin; protein engineering; aqueous two-phase systems; hydrophobic interaction chromatography;

    Sammanfattning : In recent years shortages of donated blood have become a growing problem for health care systems world-wide. Blood substitutes based on human haemoglobin (Hb) could potentially ameliorate this situation. However, clinical trials have indicated problems due to e.g. LÄS MER

  7. 5. Picture Viewing and Picture Description: Two Windows on the Mind

    Författare :Jana Holsanova; Kognitionsvetenskap; []
    Nyckelord :HUMANIORA; HUMANITIES; discourse hierarchy; picture description; vision; picture viewing; information flow; discourse segmentation; discourse analysis; coherence; semantics; spatial and temporal relations; cognitive science; linguistics; cognitive linguistics; psycholinguistics; speech; cognition; multimodal communication; mental processes; eye movement protocol; focus of attention; spoken language protocol; information processing; scene perception; areas of interest;

    Sammanfattning : In this thesis, I connect two disciplines, linguistics and vision research, and combine two methods, spoken language protocols and eye movement protocols, in order to cast light on the underlying cognitive processes. In a series of studies, I investigate the processual aspects of picture viewing and picture description. LÄS MER