Sökning: "Ganesh Eknath Phad"

Hittade 1 avhandling innehållade orden Ganesh Eknath Phad.

1. 1. Repertoire diversity and maturation of HIV-1 vaccine-induced B cell responses

   Författare :Ganesh Eknath Phad; Karolinska Institutet; Karolinska Institutet; []
   Nyckelord :;

   Sammanfattning : In the work presented in this thesis, I characterized B cell responses in rhesus macaques inoculated with HIV-1 envelope glycoprotein (Env)-based vaccines to define genetic and functional properties of the elicited antibodies for an improved understanding of Env immunogenicity. In Paper I, we investigated antibody heavy chain V gene segment expression in the overall B cell repertoire and in response to vaccination by using 2 approaches, 454-pyrosequencing and single-cell RT-PCR of bulk and sorted memory B cells, respectively. LÄS MER