Sökning: "Fed-batch cultivation"

Visar resultat 1 - 5 av 35 avhandlingar innehållade orden Fed-batch cultivation.

1. 1. Probing Control : Analysis and Design with Application to Fed-Batch Bioreactors

   Författare :Stéphane Velut; Institutionen för reglerteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Elektronik och elektroteknik; Electronics and Electrical technology; Large scale fermentations; Extremum control; Feeding strategy; Probing control; Fed-batch bioreactors; Automation; robotics; control engineering; Automatiska system; robotteknik; reglerteknik;

   Sammanfattning : In most control problems the objective is to control the output at a desired value in spite of disturbances. In some cases, the best setpoint is not known a priori and it should be found online to optimize the process performance. This thesis examines a probing strategy that can be applied for this class of problems. LÄS MER

3. 2. Improving Ethanol Production from Lignocellulose Hydrolyzates: Fed-batch Fermentation, Yeast Cultivation and Strain Development for Increased Tolerance

   Författare :Anneli Petersson; Avdelningen för kemiteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Kemiteknik och kemisk teknologi; Chemical technology and engineering; Saccharomyces cerevisiae; fed-batch control; Ethanol production; dilute acid hydrolyzate;

   Sammanfattning : Ethanol production by the yeast Saccharomyces cerevisiae from dilute acid lignocellulose hydrolyzate was studied with the aim to improve fuel ethanol production. Specifically, the problem of inhibition was addressed. LÄS MER

4. 3. Metabolic engineering and cultivation strategies for recombinant production of (R)-3-hydroxybutyrate

   Författare :Mariel Perez-Zabaleta; Gen Larsson; Antonius J. A. van Maris; Diethard Mattanovich; KTH; []
   Nyckelord :Escherichia coli; R -3-hydroxybutyrate; nitrogen limitation; nitrogen depletion; lignocellulose; fed batch; acetate; β-ketothiolase; acetoacetyl-CoA reductase; Halomonas boliviensis.; Bioteknologi; Biotechnology;

   Sammanfattning : Metabolic engineering and process engineering are two powerful disciplines to design and improve microbial processes for sustainable production of an extensive number of compounds ranging from chemicals to pharmaceuticals. The aim of this thesis was to synergistically combine these two disciplines to improve the production of a model chemical called (R)-3-hydroxybutyrate (3HB), which is a medium-value product with a stereocenter and two functional groups. LÄS MER

5. 4. Growth rate control of periplasmic product retention in Escherichia coli

   Författare :Emma Bäcklund; Gen Larsson; Stefan Schmidt; Amelie Eriksson Karlström; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Escherichia coli; fed-batch; outer membrane proteins; recombinant proteins; specific growth rate; periplasmic retention; phosphotransferase system; high cell density cultivation; acetate formation; Bioengineering; Bioteknik;

   Sammanfattning : The recombinant product is secreted to the periplasm in many processes where E. coli is used as host. One drawback with secretion is the undesired leakage of the periplasmic products to the medium. The aim of this work was to find strategies to influence the periplasmic retention of recombinant products. LÄS MER

7. 5. Bench-Scale Production of Heterologous Proteins from Extremophiles- Escherichia coli and Pichia pastoris based expression systems

   Författare :Santosh Ramchuran; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Glycoside hydrolase; Heterologous protein; High cell density cultivation Expression systems; Bioteknik; Biotechnology; Fed-batch;

   Sammanfattning : Over the past few years considerable research attention has been assigned to extremophiles as sources of extremozymes due to their applicability in industrial processes, and the development of eco-friendly technologies. The establishment of efficient production strategies for heterologous proteins is an empirical process requiring broad background knowledge on available expression systems together with their major advantages and shortcomings. LÄS MER