Sökning: "FRET"

Visar resultat 6 - 10 av 75 avhandlingar innehållade ordet FRET.

1. 6. Adsorption of biopolymers and their layer-by-layer assemblies on hydrophilic surfaces

   Författare :Maria Lundin; Eva Blomberg; Thomas Arnebrant; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Layer-by-layer; multilayer; adsorption; biopolymers; Chitosan; Heparin; Mucin; Albumin; Lysozyme; β-casein; SDS; QCM-D; Ellipsometri; DPI; TIRF; FRET; SFA; layer structure; solvent content; vertical diffusion; exponential growth; solid-liquid interface; deposition conditions; Surface and colloid chemistry; Yt- och kolloidkemi;

   Sammanfattning : It is widely known that surfaces play an important role in numerous biological processes and technological applications. Thus, being able to modify surface properties provides an opportunity to control many phenomena occurring at interfaces. LÄS MER

3. 7. Fluorescent Nucleobase Analogues and their use for Investigating DNA Interactions

   Författare :Anke Dierckx; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; FRET; B-DNA; circular dichroism; fluorescent nucleobase analogue; adenine analogue; duplex stability; Fluorescence quantum yield;

   Sammanfattning : Ever since unravelling the structure of DNA, an expanding research field has emerged with ongoing efforts dedicated to increase our understanding of the molecule of life. Since the natural nucleobases are virtually non-emissive, it has been a challenge for decades to ‘light up’ DNA/RNA in order to investigate their properties utilizing fluorescence techniques. LÄS MER

4. 8. Functional association networks for disease gene prediction

   Författare :Dimitri Guala; Erik L L Sonnhammer; Hjalmar Brismar; Yves Moreau; Stockholms universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; network biology; biological networks; network prediction; functional association; functional coupling; network integration; functional association networks; genome-wide association networks; gene networks; protein networks; fret; functional enrichment analysis; network cross-talk; pathway annotation; gene prioritisation; network-based gene prioritization; benchmarking; Biochemistry towards Bioinformatics; biokemi med inriktning mot bioinformatik;

   Sammanfattning : Mapping of the human genome has been instrumental in understanding diseasescaused by changes in single genes. However, disease mechanisms involvingmultiple genes have proven to be much more elusive. Their complexityemerges from interactions of intracellular molecules and makes them immuneto the traditional reductionist approach. LÄS MER

5. 9. DNA-Controlled Lipid-Membrane Fusion

   Författare :Lisa Simonsson; Chalmers tekniska högskola; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; exocytosis; vesicles; DNA; FRET; fluorescence resonance energy transfer; model system; total internal reflection fluorescence microscopy; TIRF microscopy; self-assembly; site-specificity; diffusion; biomembranes; lipid bilayer; membrane fusion;

   Sammanfattning : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. We have demonstrated that short DNA oligonucleotides, membrane-attached via CH in an orientation that mimics the overall zipperlike architecture of fusion-inducing proteins, induce fusion of both suspended vesicles and vesicles site-specifically tethered to SLBs. LÄS MER

7. 10. Quantitative bioimaging in single cell signaling

   Författare :Kristoffer Bernhem; Hjalmar Brismar; Anita Aperia; Lena Scott; Christian Eggeling; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Super resolution imaging; fluoresence; bioimaging; cells; FRET; cluster analysis; labeling; image analysis.;

   Sammanfattning : Imaging of cellular samples has for several hundred years been a way for scientists to investigate biological systems. With the discovery of immunofluorescence labeling in the 1940’s and later genetic fluorescent protein labeling in the 1980’s the most important part in imaging, contrast and specificity, was drastically improved. LÄS MER