Sökning: "DNA strand exchange"
Visar resultat 1 - 5 av 13 avhandlingar innehållade orden DNA strand exchange.
1. DNA strand exchange and hydrophobic interactions between biomolecules
Sammanfattning : The role of hydrophobic interactions in DNA strand exchange has been studied using fluorescence-labeled DNA oligomers in a FRET assay. Strand exchange was found to be accelerated in the presence of polyethylene glycol, which provides a crowded and hydrophobic environment possibly mimicking that of the catalytically active recombinase-DNA complexes. LÄS MER
2. Aspects of DNA Strand Exchange: Recombination Proteins and Model System Studies
Sammanfattning : DNA recombination is of fundamental importance to all living cells; it is part of the DNA repair machinery and a means to generate genetic diversity. DNA strand exchange, the exchange of strands between homologous DNA molecules, is the central reaction of the recombination process. LÄS MER
3. Structural Adaptation of DNA to Small Ligands and Recombination Proteins
Sammanfattning : In order to contribute to the understanding of mechanisms of recognition in nucleic acid systems, the interaction of DNA with a variety of ligands, representing different cases of adaptation of the double-helix structure, was studied using flow linear dichroism (LD), circular dichroism (CD), fluorescence and absorption spectroscopic techniques. A chiral hydrophobic metal complex, [Ru(dpphen)3]2+ was examined, since this had been reported to be the most efficient molecule to discriminate DNA handedness. LÄS MER
4. DNA-Complexes with Drugs and Proteins
Sammanfattning : DNA is fundamental for all living cells; the DNA holds the genetic code, which is more or less the instruction book for how all cells are built and function. Several diseases are also linked to DNA, emerging either from a mutation in our genome, which could result in a malfunctioning protein, or that the transcription of genes is somehow affected by structural changes in the DNA, induced by mutations or DNA binding molecules. LÄS MER
5. Biophysical studies of DNA binding – by the large filament-forming protein Rad51 and the small minor-groove binder Hoechst 33258
Sammanfattning : Mechanistic insight into the nature of DNA-binding ligands is crucial for both drug development as well as understanding more complex biological reactions that take place in the cell. In this Thesis, two rather different DNA-binding molecules are considered: 1. LÄS MER
