Sökning: "DNA fragmentation"

Visar resultat 1 - 5 av 75 avhandlingar innehållade orden DNA fragmentation.

1. 1. Advances in DNA Detection

   Författare :Yajing Song; Olof Emanuelsson; Jonas Tegenfeldt; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; DNA detection; active filter paper; visual detection; throughput; fluorescence; superparamagnetic beads; rare DNA; Q-PCR; chemical fragmentation; DNA library preparation; massively parallel sequencing; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Sammanfattning : DNA detection technologies have an increasing importance in our everyday lives, with applications ranging from microbial diagnostics to forensic analysis, food safety evaluation, and environmental monitoring. Currently, as the associated costs decrease, DNA diagnostic techniques are routinely used in research laboratories, in clinical and forensic practice. LÄS MER

3. 2. Electric DNA arrays for determination of pathogenic Bacillus cereus

   Författare :Yanling Liu; Sven-Olof Enfors; Helena Anderson; KTH; []
   Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Bacillus cereus; haemolysin; non-haemolytic enterotoxin; cytotoxin K-2; cereulide; toxin-coding genes; bacterial colony; electric DNA chip; ultrasonication; DNA fragmentation; Biochemistry; Biokemi;

   Sammanfattning : Silicon-based electric chip arrays were developed for characterization of Bacillus cereus with respect to the capacity to produce toxins involved in food poisoning and foodborne infections. Bacteria of the B. cereus group contain different sets of four toxins encoded by eight genes. LÄS MER

4. 3. Electric DNA chips for determination of pathogenic microorganisms

   Författare :Yanling Liu; Sven-Olof Enfors; Thomas Schweder; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; electric DNA chip array; fast determination; virulence genes; multiplexed gene detection; bacterial colony; ultrasonication; DNA fragmentation; Bacillus cereus; Biology; Biologi; Bioengineering; Bioteknik;

   Sammanfattning : Silicon-based electric DNA chip arrays were utilized to fast identify pathogenic microorganisms with respect to the capacity to produce toxins involved in foodborne poisoning and infections. Bacteria of the B. cereus and the enterohemorrhagic E. LÄS MER

5. 4. An X-ray Based Spectroscopic Study of Structure Influencing Electrons : Fragmentation, Ultrafast Charge Dynamics and Surface Composition

   Författare :Geethanjali Gopakumar; Olle Björneholm; Carl Caleman; Isaak Unger; Ruth Signorell; Uppsala universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Fragmentation; ICD; ETMD; Ultrafast electron dynamics; Surface propensity;

   Sammanfattning : The structure of biomolecules, such as proteins, is intimately connected to the function of the molecules. These structures are often studied using X-ray diffraction. However, the interaction of the X-ray photons with the molecule can excite or ionize its electrons which in turn can causes changes in the molecular structure. LÄS MER

7. 5. Oxidative damage and the DNA glycosylase MutYH

   Författare :Kristina Jansson; Göteborgs universitet; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Oxidative damage; 8-oxo-7; 8-dihydro-2′-deoxyguanosine; Base excision repair; DNA glycosylase; Mismatch repair; DNA damage checkpoint; 9-1-1 complex; Glutathione-S-transferases; Colon cancer.;

   Sammanfattning : The DNA glycosylase MutYH is highly conserved throughout evolution, and homologs are found in most eukaryotes and prokaryotes examined. MutYH functions as a base excision repair DNA glycosylase that excises adenines misincorporated opposite 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), one of the most stable products of oxidative DNA damage. LÄS MER