Sökning: "Cutinase"

Visar resultat 1 - 5 av 11 avhandlingar innehållade ordet Cutinase.

1. 1. Improving selectivity in protein extraction by subtle interactions and environmental changes

   Författare :Sheryl Fernandes; Bioteknik; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

   Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER

3. 2. Peptide-tagged proteins in aqueous two-phase systems

   Författare :Anna Nilsson; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Metabolism; Biokemi; metabolism; Biochemistry; green fluorescent protein; cutinase; tryptophan; Aqueous two-phase system; peptide-tagged protein;

   Sammanfattning : This thesis deals with proteins containing peptide tags for improved partitioning in aqueous two-phase systems. Qualitatively the peptide-tagged protein partitioning could be predicted from peptide data, i.e. partitioning trends found for peptides were also found for the peptide-tagged proteins. LÄS MER

4. 3. Integration of Aqueous Two-Phase Systems into Recovery Processes for Biomolecules

   Författare :Cecilia Kepka; Biokemi och Strukturbiologi; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Proteins; proteins; plasmid DNA; large-scale; protein purification; integration; Expression; aqueous two-phase systems; enzymology; Proteiner; enzymologi;

   Sammanfattning : The main scope of this thesis has been to study and develop new purification methods and also to further develop existing methods for purification to enhance their efficacy. Important parameters in the development of the methods are that they should be suitable for scale-up and possible to be integrated into recovery processes. LÄS MER

5. 4. Rational and combinatorial genetic engineering approaches for improved recombinant protein production and purification

   Författare :Nina Bandmann; Per-Åke Nygren; Helena Berglund; KTH; []
   Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Aqueous two-phase system; combinatorial library; expression vector; flow cytometry; fusion tag; partitioning; peptide library; phage display; recombinant proteins; ribosomal binding site; translation initiation; Bioengineering; Bioteknik;

   Sammanfattning : The bacterium Escherichia coli (E. coli) is in many situations an ideal host for production of recombinant proteins, since it generally provides a rapid and economical means to achieve sufficiently high product quantities. LÄS MER

7. 5. Lipase selectivity in functional polyester synthesis

   Författare :Cecilia Hedfors; Karl Hult; Romas Kazlauskas; KTH; []
   Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi;

   Sammanfattning : Enzyme selectivity means that the enzyme´s preferences towards competing substrates will be different. In this thesis, the enzyme selectivity has been studied for utilization in synthesis of functionalized macromonomers. LÄS MER