Sökning: "Cloning"

Visar resultat 1 - 5 av 220 avhandlingar innehållade ordet Cloning.

  1. 1. Positional Cloning of Disease Causing Genes : A Genetic Study of Obesity, Ichthyosis Prematurity Syndrome and Meniere's Disease

    Författare :Joakim Klar; Niklas Dahl; Catharina Larsson; Uppsala universitet; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Positional cloning; Obesity; Ichthyosis; Meniere’s disease; RORa; PIK3C2G; Genetik; Clinical genetics; Klinisk genetik;

    Sammanfattning : Positional cloning is a method to identify genes from their position in the genome without prior knowledge about function. We used this approach to investigate the basis for three distinct genetic disorders; Obesity, Ichthyosis Prematurity Syndrome and Meniere's disease. LÄS MER

  3. 2. CRISPR based technologies for high-throughput metabolic engineering of yeast

    Författare :Christos Skrekas; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cloning; Saccharomyces cerevisiae; enrichment; metabolic engineering; selection; library; screening; CRISPR; directed evolution;

    Sammanfattning : The yeast Saccharomyces cerevisiae is a commonly used microorganism for metabolic engineering applications since it has a very well-studied metabolism and it can be easily genetically modified. This led to its use as a cell factory for the production of a wide variety of industrially relevant chemicals such as fuels, cosmetics and food additives. LÄS MER

  4. 3. Glycoside hydrolases from extremophiles isolated in Iceland. alpha-L-Rhamnosidases and pectin degrading enzymes

    Författare :Hakon Birgisson; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; immobilization; Biotechnology; Bioteknik; crystallization; sequencing; cloning; screening; thermophile; psychrotroph; psychrophile; alpha-L-arabinofuranosidase; alpha-L-rhamnosidase; thermostable; cold active; polygalacturonase;

    Sammanfattning : Extreme environments (high and low temperature, high and low pH, etc.) are inhabited by a vast and diverse flora of microorganisms, which are adapted to these environments (extremophiles). LÄS MER

  5. 4. The cholecystokinin receptor family : molecular cloning and pharmacological characterization

    Författare :Isabelle Nilsson; Dan Larhammar; Linköpings universitet; []
    Nyckelord :MEDICINE; MEDICIN;

    Sammanfattning : Cholecystokinin (CCK) and gastrin are hormones/neurotransmittors of the gastrointestinal tract and central nervous system. The receptors for gastrin and CCK are members of the G protein-coupled receptor family. The aim of this study was to clone and pharmacologically characterize vertebrate and invertebrate CCK receptors and splice variants. LÄS MER

  7. 5. Galactoglucomannan-degrading enzymes from Aspergillus niger

    Författare :Pia Ademark; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; beta-mannanase; Aspergillus niger; beta-mannosidase; alpha-galactosidase; purification; hydrolysis; cloning; galactoglucomannan; expression; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : Galactoglucomannan and galactomannan are among the most abundant plant polysaccharides in nature. The central enzymes involved in their degradation, i.e., endo-1,4-beta-mannanase (beta-mannanase, EC 3. LÄS MER