Sökning: "Circular Dichroism Spectroscopy"

Visar resultat 1 - 5 av 66 avhandlingar innehållade orden Circular Dichroism Spectroscopy.

  1. 1. Intelligent Nucleic Acid Interactions with Peptide Nucleic Acid (PNA) and Recombination Enzyme RecA

    Författare :Pernilla Wittung; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; fluorescence; RecA; homologous recombination; circular dichroism; DNA analogs; -anti-BPDE; spectroscopy; PNA; DNA-protein interactions;

    Sammanfattning : The work presented in this thesis focuses on interactions between nucleic-acids in biological systems, particularly interactions of the promising drug-candidate peptide nucleic acid (PNA), and on protein-DNA intermediates during recombination. Several experimental techniques, mainly circular dichroism and fluorescence spectroscopy, have been used to characterize the interactions of interest. LÄS MER

  3. 2. The effect of sequence truncation and amino-acid substitutions on the biophysical properties of protein S and calbindin D9k

    Författare :Karin Julenius; Biofysikalisk kemi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; surface plasmon resonance; fluorescence spectroscopy; circular dichroism spectroscopy; NMR spectroscopy; hydrophobic effect; protein stability; dimerization; cooperativity; calcium binding; EGF-module; EF-hand; calbindin D9k; protein S; synthet; Physical chemistry; Fysikalisk kemi;

    Sammanfattning : Protein S is a modular protein and a cofactor in the protein C anticoagulant system. It consists of a Gla-module, a thrombin-sensitive loop, four EGF-modules and a SHBG-like module. LÄS MER

  4. 3. Improving selectivity in protein extraction by subtle interactions and environmental changes

    Författare :Sheryl Fernandes; Bioteknik; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Difference Spectroscopy; Circular dichroism; Biotechnology; Bioteknik; Chemical technology and engineering; Kemiteknik och kemisk teknologi; Fluorescence spectroscopy; Metal ions; Cibacron blue; Polyvinylpyrrolidone; Polyethylene glycol; Triton X-114; His 6 tag; Cold-active ß-lactamase; Cold-active ß-galactosidase; Lactate dehydrogenase; Cutinase; Affinity Extraction; Conformational change; Partial Denaturation; Molecular interaction; Aqueous two-phase systems; Detergent foam;

    Sammanfattning : The challenges for efficient bioprocesses lie in developing and adapting further current technologies or in creating innovative separation technologies. The thesis is based on the studies on protein recovery from crude feedstocks by extraction in aqueous two-phase systems and detergent foam, respectively. LÄS MER

  5. 4. Membrane Orientation Studies with Polarized Light Spectroscopy

    Författare :Malin Ardhammar; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; lipid vesicle; membrane orientation; flow linear dichroism; oriented circular dichroism;

    Sammanfattning : Lipid vesicles are versatile tools in modern biophysical science. They can be used as models of the cell membrane, to examine permeability, stability and behavior of molecules in the bilayer. They may act as transporters in a pharmacological context, designed to deliver their cargo to a certain place in the patient. LÄS MER

  7. 5. A Chloroplast Localized Small Heat Shock Protein, Hsp21. Importance of Hsp21 in stress protection of transgenic Arabidopsis thaliana, recombinant expression and characterization of oxidation-dependent conformational changes

    Författare :Ulrika Härndahl; Biokemi och Strukturbiologi; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; a-crystallin; sHsp; chaperone; amphipathic a-helix; oligomer; redox-regulated; methionine sulfoxidation; oxidative stress; regulation; protein structure; circular dichroism; mass spectrometry; fluorescence spectroscopy; Biochemistry; Metabolism; Biokemi;

    Sammanfattning : A specific group of heat shock proteins, the so called small heat shock proteins (sHsps), exists in all organisms but are especially abundant in plants. The sHsps prevent aggregation of other proteins (substrate proteins) during transient heat and oxidative stress. LÄS MER