Sökning: "Candida antarctica lipase B"

Visar resultat 1 - 5 av 51 avhandlingar innehållade orden Candida antarctica lipase B.

  1. 1. Rational redesign of Candida antarctica lipase B

    Författare :Anders Magnusson; Karl Hult; Bernhard Hauer; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; rational redesign; secondary alcohols; substrate-assisted catalysis; S-selective; entropy; aldolase; stereospecificity pocket; oxyanion hole.; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. LÄS MER

  3. 2. Expression and Mutagenesis studies of Candida antactica lipase B

    Författare :Johanna C. Rotticci-Mulder; KTH; []
    Nyckelord :Protein expression; Candida antarctica lipase B; Pichia pastoris; site-directed mutagenesis; fermentation; selection;

    Sammanfattning : Recombinant Candida antarctica lipase B was successfullyproduced in the methylotropic yeast Pichia pastoris. Thespecific activities of Candida antarctica lipase B produced inPichia pastoris and commercial Candida antarctica lipase B fromNovozymes were the same. In shake-flask cultivations theexpression levels were about 25 mg L-1. LÄS MER

  4. 3. Engineering of Candida antarctica Lipase A and B

    Författare :Johanna Löfgren; Stockholms universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : In organic chemistry, it is often desirable to have an efficient process yieldingpure product and not too much waste. When applicable, it is also desirable tohave a high enantioselective outcome. Enzymes are often displaying high regio-,chemo- and stereoselectivity. LÄS MER

  5. 4. Lipase chemoselectivity - kinetics and applications

    Författare :Cecilia Hedfors; Karl Hult; Mats Martinelle; KTH; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; Rhizomucor miehei lipase; active site titration; kinetics; chemoselectivity; thiol; alcohol; thiol-functionalized polyesters; Biochemistry; Biokemi;

    Sammanfattning :   A chemoselective catalyst is preferred in a chemical reaction where protecting groups otherwise are needed. The two lipases Candida antarctica lipase B and Rhizomucor miehei lipase showed large chemoselectivity ratios, defined as (kcat/KM)OH / (kcat/KM)SH, in a transacylation reaction with ethyl octanoate as acyl donor and hexanol or hexanethiol as acyl acceptor (paper I). LÄS MER

  7. 5. Lipase-Catalyzed Syntheses of Telechelic Polyesters

    Författare :Magnus Eriksson; Karl Hult; Mats Martinelle; Sergio Riva; KTH; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Candida antarctica lipase B; lipase-catalysis; polymerization; telechelic; functional polyesters; ring-opening polymerization; polycondensation.; Biochemistry; Biokemi;

    Sammanfattning : Telechelic polyesters have successfully been synthesized with lipase-catalyzed polymerization. The produced telechelics had a high degree of di­functionalization, high purity (requiring little or no workup) and controlled degree of polymerization. The syntheses were performed in one-pot one-step reaction systems. LÄS MER