Sökning: "Bradford Assay"

Hittade 4 avhandlingar innehållade orden Bradford Assay.

  1. 1. Design of solid dosage forms for mucosal vaccination - Investigations on the influence of excipients on product performance

    Författare :Annika Borde; Chalmers tekniska högskola; []
    Nyckelord :TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; hydrophilic matrix tablets; osmotic pressure; protein release; freeze-drying; Vibrio cholerae; mucoadhesion; Bradford Assay; sublingual vaccine tablet; mucosal vaccination;

    Sammanfattning : Most vaccines today are liquid formulations for parental administration. However, there are several drawbacks connected to these vaccines. Since injectable vaccines only induce systemic antibody responses, they are not effective against the various pathogens that affect mucosal surfaces with poor permeability for serum-derived antibodies, e.g. LÄS MER

  3. 2. Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1

    Författare :Shusheng Zuo; Bengt Mannervik; Bengt Persson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Cholesterol; Cytochalasin B; Glucose; GLUT1; Hummel and Dreyer analysis; Immobilization; PEG 5000 -DSPE; Biomembrane; Proteoliposome; Quantitative frontal affinity chromatography; Red blood cell streptavidin-biotin immobilization; Sulfhydryl affinity chromatography; The modified micro-Bradford CaPE assay; MALDI-ToF-MS; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : The human glucose transporter GLUT1 facilitates glucose to be accumulated on the other side of the cell membrane. The functional state of GLUT1 is uncertain due to diversity of the reports. In this thesis, the activity of red blood cell GLUT1 was extensively studied to further characterize this protein. LÄS MER

  4. 3. Implications of light scattering and secondary structure in protein concentration determination

    Författare :Nils Carlsson; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES;

    Sammanfattning : When using proteins in applications the amount of protein is a fundamental property. For enzyme immobilization there is a need to measure the protein concentration at a solid support. The system studied in this work is mesoporous particles. LÄS MER

  5. 4. Dentine caries : acid tolerant microorganisms and aspects on collagen degradation

    Författare :Anders Hedenbjörk Lager; Malmö högskola; []
    Nyckelord :MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Dental Caries; Collagen; Cariogenic Agents;

    Sammanfattning : Karies är en stor folksjukdom, trots att den både kan förebyggas och behandlas effektivt. Om man inte ingriper preventivt och låter det naturliga förloppet råda, kommer kariesangreppet till slut att bryta igenom emaljen och involvera även den underliggande dentinvävnaden. LÄS MER