Sökning: "ADH-A"

Hittade 5 avhandlingar innehållade ordet ADH-A.

  1. 1. Characterization and Directed Evolution of an Alcohol Dehydrogenase : A Study Towards Understanding of Three Central Aspects of Substrate Selectivity

    Författare :Emil Hamnevik; Mikael Widersten; Gerrit Poelarends; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Alcohol dehydrogenase; ADH-A; Biocatalysis; Directed evolution; Enantioselectivity; Enzyme kinetics; Enzyme mechanisms; Protein Engineering; Regioselectivity; Substrate selectivity; Biokemi; Biochemistry;

    Sammanfattning : Many different chemicals are used in the everyday life, like detergents and pharmaceuticals. However, their production has a big impact on health and environment as much of the raw materials are not renewable and the standard ways of production in many cases includes toxic and environmentally hazardous components. LÄS MER

  3. 2. Engineered Alcohol Dehydrogenases for Stereoselective Chemical Transformations

    Författare :Thilak Reddy Enugala; Mikael Widersten; Manfred T. Reetz; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; alcohol dehydrogenase-A; biocatalysts; protein engineering; enzyme kinetics; sec-alcohols; ketones; stereoselectivity; regioselectivity; substrate selectivity and promiscuity.; Biokemi; Biochemistry;

    Sammanfattning : Enzymes are biomolecules built from amino acids and catalyze the chemical transformations in a cell. Enzymes are by nature stereoselective, biodegradable, environmentally friendly, and can perform catalysis in aqueous solutions and at ambient temperatures. LÄS MER

  4. 3. Oxidation of 1,2-Diols Using Alcohol Dehydrogenases : From Kinetic Characterization to Directed Evolution

    Författare :Cecilia Blikstad; Mikael Widersten; Stefan Lutz; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; enzyme kinetics; alcohol dehydrogenase; directed evolution; enzyme engineering; diol; α-hydroxy aldehyde; Biokemi; Biochemistry;

    Sammanfattning : The use of enzymes as catalysts for chemical transformations has emerged as a “greener” alternative to traditional organic synthesis. An issue to solve though, is that enzymes are designed by nature to catalyze reactions in a living cell and therefore, in many cases, do not meet the requirements of a suitable biocatalyst. LÄS MER

  5. 4. Structure Function Relationships in Pyrimidine Degrading and Biocatalytic Enzymes, and Their Implications for Cancer Therapy and Green Chemistry

    Författare :Dirk Maurer; Doreen Dobritzsch; Mikael Widersten; Rikkert Wierenga; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; β-Ureidopropionase; Dihydropyrimidine Dehydrogenase; Alcohol Dehydrogenase; Pyrimidine catabolism; 5-Fluorouracil; CASTing; X-ray Crystallography;

    Sammanfattning : This thesis includes the work of two separate projects, studies on pyrimidine degrading enzymes and studies on in vitro evolved enzymes. The common denominator of both projects was the use of structural information to explain functional effects, observed in the studied biocatalysts. LÄS MER

  7. 5. Mutational effects on protein structure and function

    Författare :Jonas Carlsson; Persson Bengt; Uwe Sauer; Linköpings universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mutation; prediction; phenotypes; homology model; virtual screening; molecular dynamics; amyloid; cancer; membrane protein; Bioinformatics; Bioinformatik;

    Sammanfattning : In this thesis several important proteins are investigated from a structural perspective. Some of the proteins are disease related while other have important but not completely characterised functions. LÄS MER