Sökning: "α β hydrolase fold"

Visar resultat 1 - 5 av 6 avhandlingar innehållade orden α β hydrolase fold.

  1. 1. Characterization of Epoxide Hydrolases from Yeast and Potato

    Författare :Lisa Tronstad-Elfström; Mikael Widersten; Michael Arand; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Epoxide hydrolase; Catalytic residues; Active site; trans-stilbene oxide; Rapid kinetics; Active site tyrosyls; Enzyme mechanism; Lewis acid; X-ray crystallography; Substrate specificity; Unidentified ORF; α β hydrolase fold; Saccharomyces cerevisiae; Biokemi; Biochemistry; Biokemi;

    Sammanfattning : Epoxides are three-membered cyclic ethers formed in the metabolism of foreign substances and as endogenous metabolites. Epoxide hydrolases (EHs) are enzymes that catalyze the hydrolysis of epoxides to yield the corresponding diols. LÄS MER

  3. 2. Tunnels and Grooves : Structure-Function Studies in Two Disparate Enzymes

    Författare :Daniel Ericsson; Sherry Mowbray; Guy Dodson; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Mycobacterium tuberculosis; ribonucleotide reductase; peptide inhibitors; fluorescence polarization; lipase; interfacial activation; hydrolase; X-ray structure; substrate specificity; Structural biology; Strukturbiologi;

    Sammanfattning : This thesis describes structural and binding studies in enzymes from two different  organisms: ribonucleotide reductase from Mycobacterium tuberculosis (RNR) and lipase A from Candida antarctica (CalA).RNR is viable as a target for new drugs against the causative agent of tuberculosis. LÄS MER

  4. 3. Microbial Synthetic Biology, Systems Metabolic Engineering and Enzyme Engineering for Advanced Microbial Biodiesel Production with Saccharomyces cerevisiae

    Författare :Juan Octavio Valle Rodriguez; Chalmers tekniska högskola; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; protein rational engineering; wax ester synthase; hydrolase; advanced biofuel; fatty acid metabolism; directed evolution; chromosomal delta-integration;

    Sammanfattning : The continuous requirement of transportation biofuels has brought the necessity to establish alternatives permitting low-cost production of biodiesel while being environmentally friendly. Biodiesel production was achieved utilizing Saccharomyces cerevisiae by employing respective enzymes that catalyze the synthesis of fatty acid ethyl esters (FAEEs) based on fatty acyl-CoA molecules and ethanol. LÄS MER

  5. 4. Exploring Selectivity and Hysteresis : Kinetic Studies on a Potato Epoxide Hydrolase

    Författare :Diana Lindberg; Mikael Widersten; Gunnar Johansson; Richard N. Armstrong; Uppsala universitet; []
    Nyckelord :NATURVETENSKAP; NATURAL SCIENCES; Epoxide hydrolase; regioselectivity; selectivity; hysteresis; conformational changes; cooperativity; deep eutectic solvents; methylstyrene oxide; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Sammanfattning : The kinetic mechanism of an α/β hydrolase fold epoxide hydrolase from potato, StEH1, has been studied with the aims of explaining the underlying causes for enantio- and regioselectivity, both being important for product purity. Further effort has been laid upon understanding the causes of a hysteretic behavior discovered in the measurements leading to Paper I. LÄS MER

  7. 5. Structural Studies of a Xyloglucan Endotransglycosylase from Populus tremula x tremuloides and Three Conserved Hypothetical Proteins from Mycobacterium tuberculosis

    Författare :Patrik Johansson; Alwyn Jones; Sherry Mowbray; Pedro Alzari; Uppsala universitet; []
    Nyckelord :Cell and molecular biology; xyloglucan endotransglycosylase; Mycobacterium tuberculosis; epoxide hydrolase; fatty acid metabolism; fatty acid oxidation; crystallography; Cell- och molekylärbiologi;

    Sammanfattning : This thesis describes the structural studies of four different proteins from two organisms. Xyloglucan endotransglycosylases, XETs, are involved in plant cell wall expansion and remodeling by splitting and reconnecting xyloglucan-cellulose crosslinks. The first crystal structure of a XET enzyme has been determined to 1.8 Å. LÄS MER