On the adsorption behaviour of saliva and purified salivary proteins at solid/liquid interfaces

Sammanfattning: Salivary proteinaceous substances are known to play important roles in the formation of the salivary pellicle. The aim of this study was to investigate some aspects of the interfacial behaviour of selected purified salivary proteins, as well as human saliva secretions, using time resolved in situ ellipsometry. Hydrophobic methylated silica and hydrophilic pure silica were used as test substrates. Experiments were performed in vitro, preferentially in the low concentration range, with samples of fresh human whole resting saliva, parotid resting saliva and submandibular/sublingual resting saliva. The protein fractions investigated were human MUC5B, PRP-1, PRP-3 and statherin, as well as bovine submaxillary mucin (BSM). The results show that the adsorbed amount of material was found to be strongly related to the protein concentration in the range investigated for both pure proteins and secretions. Generally, for both pure proteins and secretions, higher amount of material was adsorbed on hydrophobic surfaces compared to hydrophilic ones. Comparison of the observed adsorption and calculated diffusion rates suggest initial adsorption of low molecular weight proteins/peptides. On hydrophilic surfaces the data indicate adsorption of proteins with diffusion rates corresponding to those of statherin, PRP-3 and PRP-1. MUC5B adsorbs in a later stage from both HWS and the individual secretions, due to its lower diffusion rate. On hydrophobic surfaces, adsorption rates were found to be faster than those calculated for any of the proteins, and thus smaller proteins/peptides appear to be involved. The similar adsorption rates and amounts of PRP-1 and parotid saliva (HPS) on hydrophilic surfaces may suggest that large aPRPs accounts for a substantial portion of the film forming capacity by HPS. Effects of added electrolyte could be explained by general screening effects and specific Ca2+ binding to serine phosphates in aqueous solutions, but were complex in phosphate buffer. Inter-individual differences in adsorbed amounts from HWS, HPS and HSMSLS were not found to be statistically significant.